SP-6 In Vitro Biofilm Research: Are Current Biofilm Models Acceptable (Adequate)?

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Program Overview:

It is recognized that bacteria are the main cause of pulpal and periapical disease. Aggregation of bacteria into well-established communities (biofilms), are very difficult to disinfect and eliminate from root canal systems. Root canal morphology is composed of irregular shaped canals, isthmuses, fins and apical ramifications; these canal irregularities are challenging areas to thoroughly clean and disinfect and from which to remove biofilms. Lately, many in vitro studies on root canal irrigation have been focused on various ways to eliminate biofilms. There have been different biofilm models developed for these types of studies, from mono to multi-species biofilms. There is currently no evidence that these type of models are appropriate for evaluating growth, maturity and resistance for testing in various protocols. In this lecture, several models will be presented and discussed.

At the conclusion, participants should be able to:

  •  Describe the role of bacteria in endodontic disease.
  • Describe different types of disinfection techniques.
  • List the advantages and disadvantages of current biofilm models used in in vitro studies.


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David E. Jaramillo, D.D.S.

Dr. David E. Jaramillo, DDS is an Associate Professor at Department of Endodontics, UT Health School of Dentistry. Endodontics I course director. Former associate professor of Endodontics at Loma Linda University

Disclosure: I declare that I have no proprietary, financial, or other personal interest of any nature or kind in any product, service, course, and/or company, or in any firm beneficially associated therewith, that will be discussed or considered during the proposed presentation.