S-6 Biopsy Result of Surgeries
CE Hours: 1.0
Description: Histological examination of the periradicular pathologic soft tissues excised during apical surgery intervention is usually recommended, especially in those circumstances in which a neoplastic condition is suspected. Microscopic examinations of the root tip and the surrounding soft tissue have also been used in an attempt to evaluate the causes of root canal treatment failure. However, the studies conducted up to the ‘90s were plagued by methodological limitations, such as use of unsuitable specimens, random sections instead of serial sections, lack of bacterial staining, and insufficient criteria of analysis. Consequently, the role of residual infection in causing treatment failure was grossly underestimated. In this presentation, the procedures for obtaining suitable biopsies, adequate laboratory processing and tissue sections are illustrated. It is demonstrated how the most common cause of root canal treatment failure is the presence of bacterial biofilms in the apical canal, in the intricacies of the root canal system, or in the extraradicular compartment.
Learning Objectives:
- Describe the surgical procedures for obtaining adequate biopsies.
- Recognize the limitations of previous research studies on the etiology of treatment failure.
- List the prevalent causes of root canal treatment failure.
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Domenico Ricucci, M.D., D.D.S.
Dr. Domenico Ricucci received his degree in General Medicine from “La Sapienza” University of Rome in 1982, and his DDS from the same University in 1985. Since then on he has maintained private dental practices limited to endodontics. He achieved the National Scientific qualification as full professor in the Italian higher education system in 2018. Dr. Ricucci’s primary research interest relates to pulpal and periapical tissue reactions to caries and treatment procedures, biofilms in endodontic infections, etiology of RTC treatment failure, pulp regeneration/revascularization. Since 1998 he has run his own histology laboratory and has developed considerable skills in hard tissue preparations for light microscopy.
Dr Ricucci has published 113 papers and has lectured both nationally and internationally. He has authored the Textbook and Atlas “Patologia e Clinica Endodontica”, the textbook and atlas “Endodontology. An integrated biological and clinical view”, also translated into Chinese, Russian, Japanese. He has also authored or co-authored 22 book chapters. He is recipient of the Louis I. Grossman International Award by the French Society of Endodontics in 2017, and the Louis I. Grossman Award by the American Association of Endodontists in 2023.
Disclosure(s): No financial relationships to disclose
